Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes

  • Authors:
    • Eugene Chang
    • Jung Mook Choi
    • Won Jun Kim
    • Eun-Jung Rhee
    • Ki Won Oh
    • Won-Young Lee
    • Se Eun Park
    • Sung Woo Park
    • Cheol-Young Park
  • View Affiliations

  • Published online on: May 28, 2014     https://doi.org/10.3892/mmr.2014.2278
  • Pages: 905-910
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Adiponectin and tumor necrosis factor‑α (TNF‑α) exert opposite effects on obesity‑associated inflammation and insulin signaling. The purpose of the present study was to investigate the effects of chronic TNF‑α on adiponectin levels in 3T3‑L1 adipocytes, as well as the potential reversal mechanisms. Differentiated 3T3‑L1 adipocytes were exposed to TNF‑α for three different incubation times and then to various wash‑off periods with or without mitogen‑activated protein kinase (MAPK) inhibitors. TNF‑α significantly reduced adiponectin gene expression in a dose‑ and time‑dependent manner and activated c‑Jun N‑terminal kinases (JNK), extracellular signal‑regulated kinases (ERK) and p38 MAPK. A 16 h restoration period fully reversed the decrease in adiponectin levels following 16 h treatment with TNF‑α; however, 16 h withdrawal of TNF‑α following 32 or 48 h treatment did not completely reverse the TNF‑α‑induced decrease in adiponectin levels. In 3T3‑L1 adipocytes, 32 or 48 h wash‑off periods were required following 32 or 48 h TNF‑α treatments, respectively. The pattern of ERK activation following TNF‑α exposure and removal was similar to the pattern of adiponectin expression. Furthermore, ERK1/2 inhibition accelerated the recovery of adiponectin levels compared with the levels in the untreated control adipocytes. Therefore, the inhibitory effects of TNF‑α on adiponectin levels in differentiated 3T3‑L1 cells were fully reversed following a wash‑out period equivalent to the TNF‑α treatment time, potentially through the ERK 1/2 pathway.
View Figures
View References

Related Articles

Journal Cover

August-2014
Volume 10 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chang E, Choi JM, Kim WJ, Rhee E, Oh KW, Lee W, Park SE, Park SW and Park C: Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes. Mol Med Rep 10: 905-910, 2014.
APA
Chang, E., Choi, J.M., Kim, W.J., Rhee, E., Oh, K.W., Lee, W. ... Park, C. (2014). Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes. Molecular Medicine Reports, 10, 905-910. https://doi.org/10.3892/mmr.2014.2278
MLA
Chang, E., Choi, J. M., Kim, W. J., Rhee, E., Oh, K. W., Lee, W., Park, S. E., Park, S. W., Park, C."Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes". Molecular Medicine Reports 10.2 (2014): 905-910.
Chicago
Chang, E., Choi, J. M., Kim, W. J., Rhee, E., Oh, K. W., Lee, W., Park, S. E., Park, S. W., Park, C."Restoration of adiponectin expression via the ERK pathway in TNFα-treated 3T3-L1 adipocytes". Molecular Medicine Reports 10, no. 2 (2014): 905-910. https://doi.org/10.3892/mmr.2014.2278