1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells

  • Authors:
    • Huaxin Hou
    • Danrong Li
    • Wei Jiang
    • Yan Liang
    • Donglian Chen
    • Yuanyuan Mo
  • View Affiliations

  • Published online on: June 10, 2014     https://doi.org/10.3892/mmr.2014.2307
  • Pages: 965-970
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Abstract

1,8‑dihydroxy‑3‑acetyl‑6‑methyl‑9,10 anthraquinone (DAMA) was synthesized from emodin. In the present study, the activity and the oncosis‑like mechanism of DAMA‑enhanced nasopharyngeal carcinoma (NPC) cell sensitivity to ionizing radiation was examined. The results demonstrated that DAMA has a 1.46‑fold radiosensitisation activity for nasopharyngeal carcinoma CNE 1 cells with a non‑cytotoxic concentration of 10 µg/ml DAMA combined with 2 Gy. Following treatment of DAMA combined with radiation, CNE‑1 cells revealed severe cytoplasmic swelling and vacuolization, swollen mitochondria and dilation of the nuclei without chromatin condensation, yielding a typical morphology of oncosis. Oncosis‑related gene expression of ATP synthase F0 subunit 6, chromatin modifying protein 6 and cyclophilin D mRNA increased significantly in the 8 Gy radiation group and the 2 Gy radiation combined with DAMA group. A significant decrease of ATP synthase protein 8 mRNA was observed and the levels of intracellular ATP were also reduced. In addition, the levels of intracellular Ca2+ were increased. In conclusion, DAMA is a potent radiation sensitizer in nasopharyngeal carcinoma cells and mediates its radiosensitisation via oncosis.
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August-2014
Volume 10 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hou H, Li D, Jiang W, Liang Y, Chen D and Mo Y: 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells. Mol Med Rep 10: 965-970, 2014
APA
Hou, H., Li, D., Jiang, W., Liang, Y., Chen, D., & Mo, Y. (2014). 1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells. Molecular Medicine Reports, 10, 965-970. https://doi.org/10.3892/mmr.2014.2307
MLA
Hou, H., Li, D., Jiang, W., Liang, Y., Chen, D., Mo, Y."1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells". Molecular Medicine Reports 10.2 (2014): 965-970.
Chicago
Hou, H., Li, D., Jiang, W., Liang, Y., Chen, D., Mo, Y."1,8-dihydroxy-3-acetyl-6-methyl-9,10 anthraquinone exhibits a potent radiosensitizing effect with induced oncosis in human nasopharyngeal carcinoma cells". Molecular Medicine Reports 10, no. 2 (2014): 965-970. https://doi.org/10.3892/mmr.2014.2307