Milk thistle seed extract protects rat C6 astroglial cells from acute cocaine toxicity

Badisa,Ramesh  B.; Fitch-Pye,Cheryl  A.; Agharahimi,Maryam; Palm,Donald  E.; Latinwo,Lekan  M.; Goodman,Carl  B.

doi:10.3892/mmr.2014.2524

Milk thistle seed extract protects rat C6 astroglial cells from acute cocaine toxicity

Authors:
- Ramesh B. Badisa
- Cheryl A. Fitch-Pye
- Maryam Agharahimi
- Donald E. Palm
- Lekan M. Latinwo
- Carl B. Goodman
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Affiliations: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA, Department of Biological Science, Florida State University, Tallahassee, FL 32310, USA, College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA, Department of Biological Science, Florida A&M University, Tallahassee, FL 32307, USA
Published online on: August 28, 2014 https://doi.org/10.3892/mmr.2014.2524
Pages: 2287-2292
Copyright: © Badisa et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Cocaine is a powerful addictive drug, widely abused in most Western countries. It easily reaches various domains within and outside of the central nervous system (CNS), and triggers varying levels of cellular toxicity. No pharmacological treatment is available to alleviate cocaine-induced toxicity in the cells without side-effects. Here, we discerned the role of milk thistle (MT) seed extract against cocaine toxicity. First, we investigated acute cytotoxicity induced by treatment with 2, 3 and 4 mM cocaine for 1 h in astroglial, liver and kidney cells in vitro, and then in living shrimp larvae in vivo. We showed that astroglial cells are more sensitive to cocaine than liver, kidney cells or larvae. Cocaine exposure disrupted the general architecture of astroglial cells, induced vacuolation, decreased cell viability, and depleted the glutathione (GSH) level. These changes may represent the underlying pathology of cocaine in the astrocytes. By contrast, MT pretreatment (200 µg/ml) for 30 min sustained the cell morphological features and increased both cell viability and the GSH level. Besides its protective effects, the MT extract was revealed to be non-toxic to astroglial cells, and displayed high free-radical scavenging activity. The results from this study suggest that enhanced GSH level underlies cell protection, and indicate that compounds that promote GSH synthesis in the cells may be beneficial against cocaine toxicity.

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1	National Institute on Drug Abuse. Nationwide Trends. Drug Facts (NIDA Research Report Series). NIDA DRUGPUBS; Bethesda, MD: 2011
2	Simpson DD, Joe GW, Fletcher BW, et al: A national evaluation of treatment outcomes for cocaine dependence. Arch Gen Psych. 56:507–514. 1999. View Article : Google Scholar : PubMed/NCBI
3	Weil C: The safety of bromocriptine in long-term use: a review of the literature. Curr Med Res Opin. 10:25–51. 1986. View Article : Google Scholar : PubMed/NCBI
4	Boukriche Y, Weisser I, Aubert P, et al: MRI findings in a case of late onset disulfiraminduced neurotoxicity. J Neurol. 247:714–715. 2000. View Article : Google Scholar : PubMed/NCBI
5	Madras BK, Xie Z, Lin Z, et al: Modafinil occupies dopamine and norepinephrine transporters in vivo and modulates the transporters and trace amine activity in vitro. J Pharmacol Exp Ther. 319:561–569. 2006. View Article : Google Scholar : PubMed/NCBI
6	de Leeuw van Weenen JE, Parlevliet ET, Maechler P, et al: The dopamine receptor D2 agonist bromocriptine inhibits glucose-stimulated insulin secretion by direct activation of the alpha2-adrenergic receptors in beta cells. Biochem Pharmacol. 79:1827–1836. 2010.
7	Eisenberg DM, Davis RB, Ettner SL, et al: Trends in alternative medicine use in the United States, 1990–1997 results of a follow-up national survey. JAMA. 280:1569–1575. 1998.
8	Post-White J, Ladas EJ and Kelly KM: Advances in the use of milk thistle (Silybum marianum). Integr Cancer Ther. 6:104–109. 2007. View Article : Google Scholar
9	Hernández R and Nazar E: Effect of silymarin in intrahepatic cholestasis of pregnancy (preliminary communication). Rev Chil Obstet Ginecol. 47:22–29. 1982.(In Spanish).
10	Flora K, Hahn M, Rosen H and Benner K: Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol. 93:139–143. 1998.
11	Schröder FH, Roobol MJ, Boevé ER, et al: Randomized, double-blind, placebo-controlled crossover study in men with prostate cancer and rising PSA: effectiveness of a dietary supplement. Eur Urol. 48:922–930. 2005.PubMed/NCBI
12	Badisa RB, Darling-Reed SF and Goodman CB: Cocaine induces alterations in mitochondrial membrane potential and dual cell cycle arrest in rat c6 astroglioma cells. Neurochem Res. 35:288–297. 2010. View Article : Google Scholar : PubMed/NCBI
13	Badisa RB, Goodman CB and Fitch-Pye CA: Attenuating effect of N-acetyl-L-cysteine against acute cocaine toxicity in rat C6 astroglial cells. Int J Mol Med. 32:497–502. 2013.PubMed/NCBI
14	Badisa RB, Tzakou O, Couladis M and Pilarinou E: Cytotoxic activities of some Greek Labiatae herbs. Phytother Res. 17:472–476. 2003. View Article : Google Scholar : PubMed/NCBI
15	Papini E, Bugnoli M, De Bernard M, et al: Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization of HeLa cells. Mol Microbiol. 7:323–327. 1993.
16	Patel RM and Patel NJ: In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods. J Adv Pharm Educ Res. 1:52–68. 2011.
17	Smith IK, Vierheller TL and Thorne CA: Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem. 175:408–413. 1988.PubMed/NCBI
18	Meyer BN, Ferrigni NR, Putnam JE, et al: Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med. 45:31–34. 1982. View Article : Google Scholar
19	McLaughlin JL: The brine shrimp lethality bioassay. Methods in Plant Biochemistry. Hostettmann K: 6. Academic Press; London: pp. 8–10. 1991
20	Ipsen J and Feigl P: Bancroft’s Introduction to Biostatistics. Harper and Row; New York, NY: pp. 2331970
21	Falleh H, Ksouri R, Chaieb K, et al: Phenolic composition of Cynara cardunculus L. organs and their biological activities. CR Biol. 331:372–379. 2008.
22	Kukic J, Popovic V, Petrovic S, et al: Antioxidant and antimicrobial activity of Cynara cardunculus extracts. Food Chem. 107:861–868. 2008. View Article : Google Scholar
23	Cunha-Oliveira T, Rego AC, Cardoso SM, et al: Mitochondrial dysfunction and caspase activation in rat cortical neurons treated with cocaine or amphetamine. Brain Res. 1089:44–54. 2006. View Article : Google Scholar : PubMed/NCBI
24	Cunha-Oliveira T, Rego AC, Morgadinho MT, et al: Differential cytotoxic responses of PC12 cells chronically exposed to psychostimulants or to hydrogen peroxide. Toxicology. 217:54–62. 2006. View Article : Google Scholar : PubMed/NCBI
25	Badisa RB and Goodman CB: Effect of chronic cocaine to rat C6 astroglial cells. Int J Mol Med. 30:687–692. 2012.
26	Chiu SY and Kriegler S: Neurotransmitter-mediated signaling between axons and glial cells. Glia. 11:191–200. 1994. View Article : Google Scholar : PubMed/NCBI
27	Araque A: Astrocyte-neuron signaling in the brain-implications for disease. Curr Opin Investig Drugs. 7:619–624. 2006.PubMed/NCBI
28	Deigner HP, Haberkorn U and Kinscherf R: Apoptosis modulators in the therapy of neurodegenerative diseases. Expert Opin Investig Drugs. 9:747–764. 2000. View Article : Google Scholar : PubMed/NCBI
29	LaRowe SD, Mardikian P, Malcolm R, et al: Safety and tolerability of N-acetylcysteine in cocaine dependent individuals. Am J Addict. 15:105–110. 2006. View Article : Google Scholar : PubMed/NCBI
30	Baker DA, McFarland K, Lake RW, et al: N-acetyl cysteine-induced blockade of cocaine-induced reinstatement. Ann NY Acad Sci. 1003:349–351. 2003. View Article : Google Scholar : PubMed/NCBI
31	Satel SL, Southwick SM and Gawin FH: Clinical features of cocaine-induced paranoia. Am J Psychiatry. 148:495–498. 1991. View Article : Google Scholar : PubMed/NCBI