Transforming growth factor-β inhibits IQ motif containing guanosine triphosphatase activating protein 1 expression in lung fibroblasts via the nuclear factor-κB signaling pathway

Zong,Chuanyue; Zhang,Xianlong; Xie,Ying; Cheng,Jiawen

doi:10.3892/mmr.2015.3353

Transforming growth factor-β inhibits IQ motif containing guanosine triphosphatase activating protein 1 expression in lung fibroblasts via the nuclear factor-κB signaling pathway

Authors:
- Chuanyue Zong
- Xianlong Zhang
- Ying Xie
- Jiawen Cheng
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Affiliations: Department of Anesthesiology, Huai'an Hospital Affiliated to Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an, Jiangsu 223002, P.R. China, Department of Anesthesiology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Anesthesiology, The First People's Hospital of Suqian, Suqian, Jiangsu 223800, P.R. China
Published online on: February 13, 2015 https://doi.org/10.3892/mmr.2015.3353
Pages: 442-448

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Abstract

IQ motif containing guanosine triphosphatase activating protein 1 (IQGAP1) is associated with idiopathic pulmonary fibrogenesis (IPF); however, characterization of the expression of IQGAP1 in lung fibroblasts has remained elusive. The present study therefore evaluated IQGAP1 expression in mouse and human lung fibroblasts under fibrotic conditions via western blot analysis. It was revealed that IQGAP1 expression levels were significantly decreased in lung fibroblasts isolated from bleomycin‑challenged mice than in those of control mice. Transforming growth factor‑β (TGF‑β) induced differentiation, as well as decreased expression of IQGAP1 in WI‑38 cells human lung fibroblasts. Furthermore, inhibition of nuclear factor (NF)‑κB activation restored the TGF‑β‑induced inhibition of IQGAP1 expression in WI‑38 cells. In lysophosphatidic acid (LPA)‑challenged WI‑38 cells, the expression of IQGAP1 was also decreased, while neutralized anti‑TGF‑β antibody treatment restored the LPA‑induced inhibition of IQGAP1 expression. These data indicated that TGF‑β inhibited IQGAP1 expression in lung fibroblasts via the NF‑κB signaling pathway, presenting a potential novel therapeutic target for the treatment of IPF.

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