Ku70 is essential for histone deacetylase inhibitor trichostatin A-induced apoptosis

  • Authors:
    • Jin Meng
    • Feng Zhang
    • Xu‑Tao Zhang
    • Tao Zhang
    • Yu‑Hua Li
    • Lei Fan
    • Yang Sun
    • He‑Long Zhang
    • Qi‑Bing Mei
  • View Affiliations

  • Published online on: February 16, 2015     https://doi.org/10.3892/mmr.2015.3358
  • Pages: 581-586
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Abstract

It was previously reported that the histone deacetylase inhibitor (HDACI) trichostatin A (TSA) induced B cell lymphoma 2 (Bcl‑2)‑associated X protein (Bax)‑dependent apoptosis in colorectal cancer (CRC) cells. In addition, Ku70 has been identified as a regulator of apoptosis, the mechanism of which proceeds via interacting with Bax. The aim of the present study was to investigate the role of Ku70 in TSA‑induced apoptosis in the CRC cell lines HCT116 and HT29. The results showed that TSA induced the acetylation of Ku70, which was found to be associated with increased apoptosis. In addition, TSA treatment promoted the release of Bax from its complex with Ku70. Bax was then detected to have translocated from the cytoplasm into the mitochondria, while cytochrome c was detected to have translocated from the mitochondria into the cytoplasm. Furthermore, knockdown of Ku70 using small interfering RNA decreased TSA‑induced apoptosis as well as downregulated the expression of Bax. These effects were rescued through pre‑treatment of cells with the proteasome inhibitor MG132. In conclusion, the results of the present study suggested that Ku70 acetylation mediated TSA‑induced apoptosis in CRC cells. In addition, Ku70 was found to be indispensable in TSA‑induced apoptosis due to its role in protecting Bax from proteosomal degradation.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Meng J, Zhang F, Zhang XT, Zhang T, Li YH, Fan L, Sun Y, Zhang HL and Mei QB: Ku70 is essential for histone deacetylase inhibitor trichostatin A-induced apoptosis. Mol Med Rep 12: 581-586, 2015.
APA
Meng, J., Zhang, F., Zhang, X., Zhang, T., Li, Y., Fan, L. ... Mei, Q. (2015). Ku70 is essential for histone deacetylase inhibitor trichostatin A-induced apoptosis. Molecular Medicine Reports, 12, 581-586. https://doi.org/10.3892/mmr.2015.3358
MLA
Meng, J., Zhang, F., Zhang, X., Zhang, T., Li, Y., Fan, L., Sun, Y., Zhang, H., Mei, Q."Ku70 is essential for histone deacetylase inhibitor trichostatin A-induced apoptosis". Molecular Medicine Reports 12.1 (2015): 581-586.
Chicago
Meng, J., Zhang, F., Zhang, X., Zhang, T., Li, Y., Fan, L., Sun, Y., Zhang, H., Mei, Q."Ku70 is essential for histone deacetylase inhibitor trichostatin A-induced apoptosis". Molecular Medicine Reports 12, no. 1 (2015): 581-586. https://doi.org/10.3892/mmr.2015.3358