Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Zhuang,Li; Shen,Li‑Da; Li,Kun; Yang,Run‑Xiang; Zhang,Qin‑Yong; Chen,Yun; Gao,Chun‑Lin; Dong,Chao; Bi,Qing; Tao,Jing‑Nan; Wang,Xiao‑Nan; Tian,Qing

doi:10.3892/mmr.2015.3372

Inhibition of livin expression suppresses cell proliferation and enhances chemosensitivity to cisplatin in human lung adenocarcinoma cells

Authors:
- Li Zhuang
- Li‑Da Shen
- Kun Li
- Run‑Xiang Yang
- Qin‑Yong Zhang
- Yun Chen
- Chun‑Lin Gao
- Chao Dong
- Qing Bi
- Jing‑Nan Tao
- Xiao‑Nan Wang
- Qing Tian
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Affiliations: Department of Medical Oncology, Yunnan Cancer Hospital, Kunming Medical University, Kunming, Yunnan 650118, P.R. China, Department of Cardiology, The First People's Hospital of Kunming, Yunnan 650011, P.R. China
Published online on: February 18, 2015 https://doi.org/10.3892/mmr.2015.3372
Pages: 547-552

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Abstract

Livin is a novel member of the inhibitor of apoptosis protein family that has been reported to be overexpressed in various types of human malignancy. Although several studies have demonstrated that livin may be used as an effective target for tumor therapy, few studies have investigated its role in human lung adenocarcinoma. In the present study, two different methods were used in order to investigate the tumor‑suppressing effect of livin in human lung adenocarcinoma cells. Firstly, small interfering (si)RNA technology was used to down regulate livin expression; siRNA‑mediated knockdown of livin was confirmed using reverse transcription quantitative polymerase chain reaction and western blot analysis, and cell proliferations was assessed using an MTT assay in vitro. Secondly, inhibition of livin expression was induced through the synergistic inhibitory effect between flavopiridol and tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL). Experimental results revealed that, following transfection of the livin gene‑silencing vector, the gene expression of livin was markedly decreased, SPC‑A1 cell proliferation was significantly reduced and the therapeutic effect of the chemotherapy drug cisplatin was markedly improved. This growth inhibitory effect was also observed in the flavopiridol and TRAIL combination treatment group. In the flavopiridol and TRAIL combination treatment group, the protein expression of livin was significantly reduced and the survival rate of SPC‑A1 cells was significantly lower than the flavopiridol and TRAIL single operation group. In conclusion, the RNA silencing and the synergistic inhibitory effect between flavopiridol with TRAIL was able to effectively inhibit the expression of livin, significantly decrease SPC‑A1 tumor cell proliferation and significantly enhance sensitivity to the chemotherapy drug cisplatin. These findings suggest that livin may be used as a novel target for tumor gene therapy.

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