Resveratrol protects cardiomyocytes from doxorubicin‑induced apoptosis through the AMPK/P53 pathway

  • Authors:
    • Mi‑Hua Liu
    • Xiao‑Long Lin
    • Dong‑Ming Guo
    • Yuan Zhang
    • Cong Yuan
    • Tian‑Ping Tan
    • Yu‑Dan Chen
    • Shao‑Jian Wu
    • Zu‑Feng Ye
    • Jun He
  • View Affiliations

  • Published online on: December 9, 2015     https://doi.org/10.3892/mmr.2015.4665
  • Pages: 1281-1286
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Abstract

Doxorubicin (DOX) is an efficient drug used in cancer therapy; however, it has severe cardiotoxic side effects. The aim of the present study was to investigate the effects of resveratrol on the adenosine monophosphate-activated protein kinase (AMPK)/P53 pathway in mediating DOX-induced cytotoxicity. H9c2 cells were exposed to 5 µM DOX for 24 h to establish a model of DOX-induced cardiotoxicity. DOX administration amplified P53 and B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) expression and decreased Bcl-2 expression in H9c2 cells. Resveratrol increased the cell viability and decreased the apoptotic rate. In addition, resveratrol markedly increased the phosphorylation of AMPK. Of note, resveratrol protected against DOX-induced increases of P53 and Bax and also prevented the downregulation of Bcl-2 in H9c2 cells. Furthermore, AMPK inhibitor Compound C abolished the protective effects of resveratrol. The results of the present study therefore indicated that resveratrol protected H9c2 cells from DOX-induced apoptosis via the AMPK/P53 pathway.
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February-2016
Volume 13 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu MH, Lin XL, Guo DM, Zhang Y, Yuan C, Tan TP, Chen YD, Wu SJ, Ye ZF, He J, He J, et al: Resveratrol protects cardiomyocytes from doxorubicin‑induced apoptosis through the AMPK/P53 pathway. Mol Med Rep 13: 1281-1286, 2016.
APA
Liu, M., Lin, X., Guo, D., Zhang, Y., Yuan, C., Tan, T. ... He, J. (2016). Resveratrol protects cardiomyocytes from doxorubicin‑induced apoptosis through the AMPK/P53 pathway. Molecular Medicine Reports, 13, 1281-1286. https://doi.org/10.3892/mmr.2015.4665
MLA
Liu, M., Lin, X., Guo, D., Zhang, Y., Yuan, C., Tan, T., Chen, Y., Wu, S., Ye, Z., He, J."Resveratrol protects cardiomyocytes from doxorubicin‑induced apoptosis through the AMPK/P53 pathway". Molecular Medicine Reports 13.2 (2016): 1281-1286.
Chicago
Liu, M., Lin, X., Guo, D., Zhang, Y., Yuan, C., Tan, T., Chen, Y., Wu, S., Ye, Z., He, J."Resveratrol protects cardiomyocytes from doxorubicin‑induced apoptosis through the AMPK/P53 pathway". Molecular Medicine Reports 13, no. 2 (2016): 1281-1286. https://doi.org/10.3892/mmr.2015.4665