MicroRNA‑26b is upregulated in a double transgenic mouse model of Alzheimer's disease and promotes the expression of amyloid‑β by targeting insulin‑like growth factor 1

  • Authors:
    • Hong Liu
    • Wenzheng Chu
    • Li Gong
    • Xiaoyu Gao
    • Wei Wang
  • View Affiliations

  • Published online on: February 4, 2016     https://doi.org/10.3892/mmr.2016.4860
  • Pages: 2809-2814
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Abstract

Alzheimer's disease (AD) is the most common form of dementia among the aging population. It is pathologically characterized by synaptic impairment, accumulation of neurofibrillary tangles and amyloid‑β (Aβ) deposition. MicroRNA‑26b (miR‑26b) has been observed to be upregulated in the human temporal cortex in AD, however, the function of miR‑26b has not been verified. Reverse transcription‑quantitative polymerase chain reaction was conducted to investigate the expression levels of miR‑26b in a double transgenic mouse model of AD. Following transfection of miR‑26b or an miR‑26b inhibitor, western blot analysis, enzyme‑linked immunosorbent assay and luciferase assays were performed. The present study demonstrated that the expression levels of miR‑26b were upregulated in a double transgenic mouse model of AD. It was also demonstrated that upregulation of miR‑26b in N2a/APP cells downregulated the insulin‑like growth factor 1 (IGF‑1) protein expression level and promoted Aβ production, whereas inhibition of miR‑26b in N2a/APP cells upregulated the IGF‑1 protein level and suppressed Aβ production. Furthermore, miR‑26b target sites in IGF‑1 were confirmed using a luciferase assay in HEK293 cells. The present study may be useful in the development of effective therapeutic strategies against AD.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu H, Chu W, Gong L, Gao X and Wang W: MicroRNA‑26b is upregulated in a double transgenic mouse model of Alzheimer's disease and promotes the expression of amyloid‑β by targeting insulin‑like growth factor 1. Mol Med Rep 13: 2809-2814, 2016
APA
Liu, H., Chu, W., Gong, L., Gao, X., & Wang, W. (2016). MicroRNA‑26b is upregulated in a double transgenic mouse model of Alzheimer's disease and promotes the expression of amyloid‑β by targeting insulin‑like growth factor 1. Molecular Medicine Reports, 13, 2809-2814. https://doi.org/10.3892/mmr.2016.4860
MLA
Liu, H., Chu, W., Gong, L., Gao, X., Wang, W."MicroRNA‑26b is upregulated in a double transgenic mouse model of Alzheimer's disease and promotes the expression of amyloid‑β by targeting insulin‑like growth factor 1". Molecular Medicine Reports 13.3 (2016): 2809-2814.
Chicago
Liu, H., Chu, W., Gong, L., Gao, X., Wang, W."MicroRNA‑26b is upregulated in a double transgenic mouse model of Alzheimer's disease and promotes the expression of amyloid‑β by targeting insulin‑like growth factor 1". Molecular Medicine Reports 13, no. 3 (2016): 2809-2814. https://doi.org/10.3892/mmr.2016.4860