Effect of bisphenol A on SOCS-3 and insulin signaling transduction in 3T3-L1 adipocytes

  • Authors:
    • Yue‑E Dai
    • Wei Chen
    • Humin Qi
    • Qian‑Qi Liu
  • View Affiliations

  • Published online on: May 9, 2016     https://doi.org/10.3892/mmr.2016.5224
  • Pages: 331-336
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Abstract

The aim of the present study was to investigate whether environmental endocrine disrupting chemical, bisphenol A (BPA), affects secretion of suppressor of cytokine signaling 3 (SOCS-3) and insulin signaling transduction in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated for 0, 2, 6, 12 and 24 h with BPA at 80 µM in serum‑deprived medium. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to detect the mRNA expression levels of SOCS‑3 and protein expression levels of SOCS‑3, insulin receptor substrate 1 (IRS‑1), phosphorylated (p)‑IRS‑1, Akt and p‑Akt. The levels of p‑IRS‑1, Akt and p‑Akt in cultures treated for 6 h with BPA were also analyzed by immunofluorescence. The SOCS‑3 mRNA and protein expression levels were decreased in the 6, 12 and 24 h groups. The levels of p‑IRS‑1 and p‑Akt protein were markedly downregulated, while the level of IRS‑1 and Akt protein remained unaltered among these groups, which was consistent with the results observed using immunofluorescence. BPA may inhibit insulin signal transduction and result in the occurrence of insulin resistance via promoting the expression of SOCS-3.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Dai YE, Chen W, Qi H and Liu QQ: Effect of bisphenol A on SOCS-3 and insulin signaling transduction in 3T3-L1 adipocytes. Mol Med Rep 14: 331-336, 2016.
APA
Dai, Y., Chen, W., Qi, H., & Liu, Q. (2016). Effect of bisphenol A on SOCS-3 and insulin signaling transduction in 3T3-L1 adipocytes. Molecular Medicine Reports, 14, 331-336. https://doi.org/10.3892/mmr.2016.5224
MLA
Dai, Y., Chen, W., Qi, H., Liu, Q."Effect of bisphenol A on SOCS-3 and insulin signaling transduction in 3T3-L1 adipocytes". Molecular Medicine Reports 14.1 (2016): 331-336.
Chicago
Dai, Y., Chen, W., Qi, H., Liu, Q."Effect of bisphenol A on SOCS-3 and insulin signaling transduction in 3T3-L1 adipocytes". Molecular Medicine Reports 14, no. 1 (2016): 331-336. https://doi.org/10.3892/mmr.2016.5224