Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation

  • Authors:
    • Xi Chen
    • Hui Zhang
    • Wenjun Mou
    • Zhan Qi
    • Xiaoya Ren
    • Guoliang Wang
    • Hong Jiao
    • Xiaohui Kong
    • Jingang Gui
  • View Affiliations

  • Published online on: September 26, 2016     https://doi.org/10.3892/mmr.2016.5780
  • Pages: 4301-4308
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Abstract

Flow cytometric analysis is important for the investigation and clinical preparation of lymphocytes from children. However, the strict requirement of cell freshness and inter‑assay variability limits the application of this methodology for pediatric investigations. Therefore, it is necessary to identify a reliable cryopreservative method capable of maintaining high cell viability and proper cell function in lymphocytes from children. In the present study, eight commonly‑used cell cyropreservative methods were used, and their effects on cell viability, surface marker expression and cell function were examined. In addition, how these methods affect the distribution of T‑cell receptor Vβ subfamilies were also determined. The results of the present study provided valuable experimental evidence, based on which the optimal method for the cryopreservation of lymphocytes from children in pediatric investigations and clinical applications can be selected.
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November-2016
Volume 14 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen X, Zhang H, Mou W, Qi Z, Ren X, Wang G, Jiao H, Kong X and Gui J: Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation. Mol Med Rep 14: 4301-4308, 2016.
APA
Chen, X., Zhang, H., Mou, W., Qi, Z., Ren, X., Wang, G. ... Gui, J. (2016). Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation. Molecular Medicine Reports, 14, 4301-4308. https://doi.org/10.3892/mmr.2016.5780
MLA
Chen, X., Zhang, H., Mou, W., Qi, Z., Ren, X., Wang, G., Jiao, H., Kong, X., Gui, J."Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation". Molecular Medicine Reports 14.5 (2016): 4301-4308.
Chicago
Chen, X., Zhang, H., Mou, W., Qi, Z., Ren, X., Wang, G., Jiao, H., Kong, X., Gui, J."Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation". Molecular Medicine Reports 14, no. 5 (2016): 4301-4308. https://doi.org/10.3892/mmr.2016.5780