Association of SCNN1B promoter methylation with essential hypertension

  • Authors:
    • Qilong Zhong
    • Chunyan Liu
    • Rui Fan
    • Shiwei Duan
    • Xuting Xu
    • Jinshun Zhao
    • Shuqi Mao
    • Wen Zhu
    • Lingmei Hao
    • Fengying Yin
    • Lina Zhang
  • View Affiliations

  • Published online on: October 31, 2016     https://doi.org/10.3892/mmr.2016.5905
  • Pages: 5422-5428
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Abstract

The amiloride-sensitive sodium channel beta subunit (SCNN1B) gene encodes the beta subunit of the epithelial sodium channel, which is involved in blood pressure homeostasis. The aim of the present study was to investigate the association between SCNN1B gene promoter methylation and essential hypertension (EH), and to explore whether SCNN1B methylation was altered by antihypertensive therapy. The present study recruited 282 individuals: 94 controls, 94 incident cases and 94 prevalent cases. Subsequently, the methylation status of six CpG sites in the SCNN1B promoter region was measured using bisulfite pyrosequencing technology. Among the six CpG sites, a significant difference in CpG1 and CpG2 methylation levels were detected between controls and incident cases (CpG1: β‑standardized=0.17, adjusted P=0.015; CpG2: β‑standardized=‑0.41, adjusted P=0.001). In addition, a significant difference was detected in CpG1 methylation levels between incident cases and prevalent cases (β‑standardized=‑0.252, adjusted P=3.77E‑04). The present study also demonstrated that CpG1 and CpG2 methylation levels were significantly lower in males compared with in females (CpG1: t=‑3.180, P=0.002; CpG2: t=‑2.148, P=0.033). CpG1 methylation was also shown to be positively correlated with age (controls: r=0.285, P=0.008; incident cases: r=0.401, P=0.0001; prevalent cases: r=0.367, P=0.001). These results indicated a significant association between EH and SCNN1B methylation, which was affected by age, gender and antihypertensive therapy.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhong Q, Liu C, Fan R, Duan S, Xu X, Zhao J, Mao S, Zhu W, Hao L, Yin F, Yin F, et al: Association of SCNN1B promoter methylation with essential hypertension. Mol Med Rep 14: 5422-5428, 2016.
APA
Zhong, Q., Liu, C., Fan, R., Duan, S., Xu, X., Zhao, J. ... Zhang, L. (2016). Association of SCNN1B promoter methylation with essential hypertension. Molecular Medicine Reports, 14, 5422-5428. https://doi.org/10.3892/mmr.2016.5905
MLA
Zhong, Q., Liu, C., Fan, R., Duan, S., Xu, X., Zhao, J., Mao, S., Zhu, W., Hao, L., Yin, F., Zhang, L."Association of SCNN1B promoter methylation with essential hypertension". Molecular Medicine Reports 14.6 (2016): 5422-5428.
Chicago
Zhong, Q., Liu, C., Fan, R., Duan, S., Xu, X., Zhao, J., Mao, S., Zhu, W., Hao, L., Yin, F., Zhang, L."Association of SCNN1B promoter methylation with essential hypertension". Molecular Medicine Reports 14, no. 6 (2016): 5422-5428. https://doi.org/10.3892/mmr.2016.5905