Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma

  • Authors:
    • Jian‑Feng Fang
    • Hai‑Ping Zhao
    • Zheng‑Fei Wang
    • Shu‑Sen Zheng
  • View Affiliations

  • Published online on: March 14, 2017     https://doi.org/10.3892/mmr.2017.6320
  • Pages: 2720-2726
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Abstract

RAS protein activator like 2 (RASAL2) has been reported to be dysregulated in various types of cancer. It has previously been demonstrated that RASAL2 is hypomethylated in hepatocellular carcinoma (HCC). However, the expression pattern of RASAL2 and its potential role in HCC remain to be elucidated. The present study demonstrated that the expression of RASAL2 was upregulated in HCC tissues, compared with in normal liver tissues, by using immunohistochemistry. In addition, Cell Counting Kit-8 assay and invasion assay revealed that knockdown of RASAL2 inhibited the growth and invasion of HCC cells. Western blotting results indicated that the inhibition of RASAL2 reduced the levels of phosphorylated-AKT. Notably, RASAL2 was observed to be a direct target of miR-203 in HCC in luciferase activity assays. Furthermore, overexpression of miR-203 exhibited a similar effect to RASAL2 knockdown in HCC cells. These results indicated that RASAL2 serves a tumor oncogenic role in HCC and may be considered a potential target in HCC.
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May-2017
Volume 15 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Fang JF, Zhao HP, Wang ZF and Zheng SS: Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma. Mol Med Rep 15: 2720-2726, 2017
APA
Fang, J., Zhao, H., Wang, Z., & Zheng, S. (2017). Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma. Molecular Medicine Reports, 15, 2720-2726. https://doi.org/10.3892/mmr.2017.6320
MLA
Fang, J., Zhao, H., Wang, Z., Zheng, S."Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma". Molecular Medicine Reports 15.5 (2017): 2720-2726.
Chicago
Fang, J., Zhao, H., Wang, Z., Zheng, S."Upregulation of RASAL2 promotes proliferation and metastasis, and is targeted by miR-203 in hepatocellular carcinoma". Molecular Medicine Reports 15, no. 5 (2017): 2720-2726. https://doi.org/10.3892/mmr.2017.6320