Open Access

Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention

  • Authors:
    • Zhi-Ming Tang
    • Xiao-Xiang Zhai
    • Ji-Cun Ding
  • View Affiliations

  • Published online on: March 16, 2017     https://doi.org/10.3892/mmr.2017.6339
  • Pages: 2546-2550
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to examine the expression of mammalian target of rapamycin (mTOR)/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol (Res) intervention. The mTOR and 70S6K in pathological scar and normal skin fibroblasts were detected by immunofluorescence following treatment with different concentrations of Res. RT-PCR and western blot analysis were used to detect the expression of mTOR and 70S6K mRNA and protein, respectively. Immunofluorescence showed that the expression of 70S6K and mTOR was significantly enhanced in pathological scar fibroblasts, and mainly expressed in the nucleus, but not in normal skin fibroblasts. RT-PCR and western blot analysis showed that after different concentrations of Res treatments, the mTOR and 70S6K mRNA and protein expression significantly (P<0.05) decreased in a dose‑dependent manner. In conclusion, the expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts was significantly enhanced. Res can downregulate the expression of mTOR and 70S6K to achieve the inhibition of pathological scar fibroblast proliferation.
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May-2017
Volume 15 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tang Z, Zhai X and Ding J: Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention. Mol Med Rep 15: 2546-2550, 2017
APA
Tang, Z., Zhai, X., & Ding, J. (2017). Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention. Molecular Medicine Reports, 15, 2546-2550. https://doi.org/10.3892/mmr.2017.6339
MLA
Tang, Z., Zhai, X., Ding, J."Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention". Molecular Medicine Reports 15.5 (2017): 2546-2550.
Chicago
Tang, Z., Zhai, X., Ding, J."Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention". Molecular Medicine Reports 15, no. 5 (2017): 2546-2550. https://doi.org/10.3892/mmr.2017.6339