Open Access

AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation

  • Authors:
    • Kunqiu Yang
    • Jiuheng Yin
    • Baifa Sheng
    • Qimeng Wang
    • Bin Han
    • Aimin Pu
    • Min Yu
    • Lihua Sun
    • Weidong Xiao
    • Hua Yang
  • View Affiliations

  • Published online on: March 23, 2017     https://doi.org/10.3892/mmr.2017.6368
  • Pages: 3019-3026
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Keratinocyte growth factor (KGF) stimulates intestinal epithelial cell proliferation upon binding to the KGF receptor (KGFR). The activated aryl hydrocarbon receptor (AhR) serves an important role in the development of tissues by promoting the expression of AhR receptors, which can regulate cell proliferation. In the present study, the signaling pathway between AhR and KGFR in investigated with regards to KGF‑induced intestinal epithelial cell proliferation. Male C57BL/6J wild type and AhR‑/‑ mice, were randomized into four groups: Control, KGF, AhR‑/‑ + KGF and AhR‑/‑ (n=6 per group). The small bowel was harvested on day 5 post‑treatment. LoVo cells were used to study signaling pathways in vitro and were divided into the following four treatment groups: DMSO, KGF, KGF + small‑interfering (si)AhR and siAhR. In vivo, knockdown of AhR mRNA transcripts may abolish KGF‑induced intestinal epithelial cell proliferation. Furthermore, KGFR expression was downregulated following knockdown or silencing of AhR expression in vivo and in vitro. The present study identified that the transcription factor E2F1 could regulate KGFR expression, and that siAhR treatment led to reduced expression of E2F1 in the nucleus and inhibited KGF‑induced cell proliferation. In conclusion, the current results demonstrated that the AhR‑E2F1‑KGFR pathway is involved in KGF‑induced intestinal epithelial cell proliferation.
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May-2017
Volume 15 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yang K, Yin J, Sheng B, Wang Q, Han B, Pu A, Yu M, Sun L, Xiao W, Yang H, Yang H, et al: AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation. Mol Med Rep 15: 3019-3026, 2017
APA
Yang, K., Yin, J., Sheng, B., Wang, Q., Han, B., Pu, A. ... Yang, H. (2017). AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation. Molecular Medicine Reports, 15, 3019-3026. https://doi.org/10.3892/mmr.2017.6368
MLA
Yang, K., Yin, J., Sheng, B., Wang, Q., Han, B., Pu, A., Yu, M., Sun, L., Xiao, W., Yang, H."AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation". Molecular Medicine Reports 15.5 (2017): 3019-3026.
Chicago
Yang, K., Yin, J., Sheng, B., Wang, Q., Han, B., Pu, A., Yu, M., Sun, L., Xiao, W., Yang, H."AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation". Molecular Medicine Reports 15, no. 5 (2017): 3019-3026. https://doi.org/10.3892/mmr.2017.6368