Open Access

AZGP1 as a potential biomarker of IgA vasculitis with nephritis in a children‑based urinary proteomics study by diaPASEF

  • Authors:
    • Zhi-Qing Zhu
    • Tian Zhang
    • Shuo Chang
    • Zhen-Hua Ren
    • Qin Zhang
  • View Affiliations

  • Published online on: June 30, 2023     https://doi.org/10.3892/mmr.2023.13044
  • Article Number: 157
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to screen for potential biomarkers in the urine of immunoglobulin A vasculitis with nephritis (IgAVN) using parallel accumulation‑serial fragmentation combined with data‑independent acquisition (diaPASEF) proteomic approach. Urine proteomes of eight children with IgAVN and eight healthy children were identified by diaPASEF and all differential proteins were analyzed by Gene Ontology and Kyoto Encyclopedia of Gene and Genome. Then, the specific biomarkers of urine samples from 10 children with IgAVN, 10 children with IgAV and 10 healthy children were verified by ELISA. The present study screened 254 differential proteins from the experiment, including 190 upregulated proteins and 64 downregulated proteins. The results of the ELISA showed that the concentration of urinary zinc‑alpha‑2‑glycoprotein (AZGP1) in children with IgAVN was significantly higher compared with that in children with IgAV and healthy children. The present study provided the potential clinical application of AZGP1 as a helpful biomarker and a potential indicator for early diagnosis of the occurrence of IgAVN.
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August-2023
Volume 28 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu Z, Zhang T, Chang S, Ren Z and Zhang Q: AZGP1 as a potential biomarker of IgA vasculitis with nephritis in a children‑based urinary proteomics study by diaPASEF. Mol Med Rep 28: 157, 2023.
APA
Zhu, Z., Zhang, T., Chang, S., Ren, Z., & Zhang, Q. (2023). AZGP1 as a potential biomarker of IgA vasculitis with nephritis in a children‑based urinary proteomics study by diaPASEF. Molecular Medicine Reports, 28, 157. https://doi.org/10.3892/mmr.2023.13044
MLA
Zhu, Z., Zhang, T., Chang, S., Ren, Z., Zhang, Q."AZGP1 as a potential biomarker of IgA vasculitis with nephritis in a children‑based urinary proteomics study by diaPASEF". Molecular Medicine Reports 28.2 (2023): 157.
Chicago
Zhu, Z., Zhang, T., Chang, S., Ren, Z., Zhang, Q."AZGP1 as a potential biomarker of IgA vasculitis with nephritis in a children‑based urinary proteomics study by diaPASEF". Molecular Medicine Reports 28, no. 2 (2023): 157. https://doi.org/10.3892/mmr.2023.13044