Open Access

LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts

  • Authors:
    • Demin Lv
    • Bingxia Li
    • Zhen Liu
    • Qing Zhang
    • Sucheng Cao
    • Yanlong Xu
    • Zheng Zhang
  • View Affiliations

  • Published online on: October 15, 2024     https://doi.org/10.3892/mmr.2024.13365
  • Article Number: 241
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Addressing the repair and regeneration of large bone defects poses significant challenges in bone tissue engineering. Despite the abundant evidence demonstrating the positive role of MSCs in osteogenesis, their limited osteogenic differentiation ability still needs to be improved. The present study used lipopolysaccharide (LPS) to enhance the osteogenic properties of ecto‑mesenchymal stem cells (EMSCs). Human nasal respiratory mucosa‑derived EMSCs were cultured on plates and stimulated with LPS for 5 days prior to undergoing osteogenic differentiation. The findings revealed that LPS effectively stimulated the osteogenic differentiation capacity of EMSCs, as evidenced by heightened alkaline phosphatase activity, elevated expression levels of osteogenic‑related proteins and enhanced mineralization of EMSCs. The present study also demonstrated that the augmentation occurred due to increased IL‑10 levels, although it was not solely attributable to this factor. Together, the findings illustrated that the LPS‑mediated adaptation of EMSCs is an active process driving osteogenic differentiation and could be a novel strategy for bone regeneration.
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December-2024
Volume 30 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Lv D, Li B, Liu Z, Zhang Q, Cao S, Xu Y and Zhang Z: LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts. Mol Med Rep 30: 241, 2024.
APA
Lv, D., Li, B., Liu, Z., Zhang, Q., Cao, S., Xu, Y., & Zhang, Z. (2024). LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts. Molecular Medicine Reports, 30, 241. https://doi.org/10.3892/mmr.2024.13365
MLA
Lv, D., Li, B., Liu, Z., Zhang, Q., Cao, S., Xu, Y., Zhang, Z."LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts". Molecular Medicine Reports 30.6 (2024): 241.
Chicago
Lv, D., Li, B., Liu, Z., Zhang, Q., Cao, S., Xu, Y., Zhang, Z."LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts". Molecular Medicine Reports 30, no. 6 (2024): 241. https://doi.org/10.3892/mmr.2024.13365