Open Access

Calycosin‑7‑O‑β‑D‑glucoside downregulates mitophagy by mitigating mitochondrial fission to protect HT22 cells from oxygen‑glucose deprivation/reperfusion‑induced injury

  • Authors:
    • Xiangli Yan
    • Siqi Quan
    • Roujia Guo
    • Zibo Li
    • Ming Bai
    • Baoying Wang
    • Pan Su
    • Erping Xu
    • Yucheng Li
  • View Affiliations

  • Published online on: January 13, 2025     https://doi.org/10.3892/mmr.2025.13436
  • Article Number: 71
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Calycosin‑7‑O‑β‑D‑glucoside (CG), a major active ingredient of Astragali Radix, exerts neuroprotective effects against cerebral ischemia; however, whether the effects of CG are associated with mitochondrial protection remains unclear. The present study explored the role of CG in improving mitochondrial function in a HT22 cell model of oxygen‑glucose deprivation/reperfusion (OGD/R). The Cell Counting Kit‑8 assay, flow cytometry, immunofluorescence and western blotting were performed to investigate the effects of CG on mitochondrial function. The results demonstrated that mitochondrial function was restored after treatment with CG, as indicated by reduced mitochondrial reactive oxygen species levels, increased mitochondrial membrane potential and improved mitochondrial morphology. Overactivated mitophagy was revealed to be inhibited by the regulation of proteins involved in fission [phosphorylated‑dynamin‑related protein 1 (Drp1) and Drp1] and mitophagy (LC3, p62 and translocase of outer mitochondrial membrane 20), and mitochondrial biogenesis was demonstrated to be enhanced by increased levels of sirtuin 1 (SIRT1) and peroxisome proliferator‑activated receptor γ coactivator‑1α (PGC‑1α). In addition, neuronal apoptosis was ameliorated by CG, as determined by a decreased rate of apoptosis, and levels of caspase‑3 and Bcl‑2/Bax. In conclusion, the present study demonstrated that CG may alleviate OGD/R‑induced injury by upregulating SIRT1 and PGC‑1α protein expression, and reducing excessive mitochondrial fission and overactivation of mitophagy.
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March-2025
Volume 31 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yan X, Quan S, Guo R, Li Z, Bai M, Wang B, Su P, Xu E and Li Y: Calycosin‑7‑O‑β‑D‑glucoside downregulates mitophagy by mitigating mitochondrial fission to protect HT22 cells from oxygen‑glucose deprivation/reperfusion‑induced injury. Mol Med Rep 31: 71, 2025.
APA
Yan, X., Quan, S., Guo, R., Li, Z., Bai, M., Wang, B. ... Li, Y. (2025). Calycosin‑7‑O‑β‑D‑glucoside downregulates mitophagy by mitigating mitochondrial fission to protect HT22 cells from oxygen‑glucose deprivation/reperfusion‑induced injury. Molecular Medicine Reports, 31, 71. https://doi.org/10.3892/mmr.2025.13436
MLA
Yan, X., Quan, S., Guo, R., Li, Z., Bai, M., Wang, B., Su, P., Xu, E., Li, Y."Calycosin‑7‑O‑β‑D‑glucoside downregulates mitophagy by mitigating mitochondrial fission to protect HT22 cells from oxygen‑glucose deprivation/reperfusion‑induced injury". Molecular Medicine Reports 31.3 (2025): 71.
Chicago
Yan, X., Quan, S., Guo, R., Li, Z., Bai, M., Wang, B., Su, P., Xu, E., Li, Y."Calycosin‑7‑O‑β‑D‑glucoside downregulates mitophagy by mitigating mitochondrial fission to protect HT22 cells from oxygen‑glucose deprivation/reperfusion‑induced injury". Molecular Medicine Reports 31, no. 3 (2025): 71. https://doi.org/10.3892/mmr.2025.13436