Anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), SOD or catalase on antimycin A-induced HeLa cell death

  • Authors:
    • Yong Hwan Han
    • Suhn Hee Kim
    • Sung Zoo Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/mmr_00000101
  • Pages: 307-311
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Abstract

Antimycin A (AMA) is an inhibitor of the electron transport chain in mitochondria. In this study, we investigated the anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), superoxide dismutase (SOD) or catalase on AMA-induced HeLa cell death in relation to the cell cycle. Treatment with Z-VAD, SOD or catalase rescued some HeLa cells from AMA-induced apoptosis, but did not prevent the growth inhibition of HeLa cells by AMA. DNA flow cytometric analysis indicated that treatment with AMA significantly induced an S-phase arrest of the cell cycle at 72 h. Interestingly, Z-VAD, SOD and catalase intensified S-phase arrest in AMA-treated cells. In conclusion, treatment with Z-VAD, SOD or catalase decreased apoptotic levels in AMA-treated cells, which was associated with the enhancement of the S-phase arrest of the cell cycle in these cells.

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March-April 2009
Volume 2 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Han YH, Kim SH, Kim SZ and Park WH: Anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), SOD or catalase on antimycin A-induced HeLa cell death . Mol Med Rep 2: 307-311, 2009.
APA
Han, Y.H., Kim, S.H., Kim, S.Z., & Park, W.H. (2009). Anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), SOD or catalase on antimycin A-induced HeLa cell death . Molecular Medicine Reports, 2, 307-311. https://doi.org/10.3892/mmr_00000101
MLA
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), SOD or catalase on antimycin A-induced HeLa cell death ". Molecular Medicine Reports 2.2 (2009): 307-311.
Chicago
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Anti-apoptotic effects of pan-caspase inhibitor (Z-VAD), SOD or catalase on antimycin A-induced HeLa cell death ". Molecular Medicine Reports 2, no. 2 (2009): 307-311. https://doi.org/10.3892/mmr_00000101