Open Access

Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts

  • Authors:
    • Bing Wang
    • Hongbo Li
    • Rui Yang
    • Shunchang Zhou
    • Shengquan Zou
  • View Affiliations

  • Published online on: September 4, 2014     https://doi.org/10.3892/ol.2014.2499
  • Pages: 1919-1924
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Decitabine (DAC), an inhibitor of DNA methyltransferase, demonstrates antitumor activities in various types of cancer. However, its therapeutic potential for cholangiocarcinoma (CCA), one of the most aggressive gastrointestinal malignancies, remains to be explored. The present study investigated the antiproliferative effects of DAC on CCA cells in vitro and in vivo. Human CCA cell lines, TFK‑1 and QBC939, were used as models to investigate DAC on the cell growth and proliferation of CCA. Cell proliferation was evaluated by Cell Counting Kit‑8 assay combined with clonogenic survival assay. Flow cytometry, Hoechst 33342/propidium iodide staining and green fluorescent protein‑tagged MAP‑LC3 detection were applied to determine cell cycle progression, apoptosis and autophagy. Nude mice with TFK‑1 xenografts were evaluated for tumor growth following DAC treatment. DAC was observed to significantly suppress the proliferation of cultured TFK‑1 and QBC939 cells, accompanied with enhanced apoptosis, autophagy and cell cycle arrest at G2/M phase. In TFK‑1 mouse xenografts, DAC retarded the tumor growth and increased the survival of CCA tumor‑bearing mice.
View Figures
View References

Related Articles

Journal Cover

November-2014
Volume 8 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang B, Li H, Yang R, Zhou S and Zou S: Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts. Oncol Lett 8: 1919-1924, 2014.
APA
Wang, B., Li, H., Yang, R., Zhou, S., & Zou, S. (2014). Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts. Oncology Letters, 8, 1919-1924. https://doi.org/10.3892/ol.2014.2499
MLA
Wang, B., Li, H., Yang, R., Zhou, S., Zou, S."Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts". Oncology Letters 8.5 (2014): 1919-1924.
Chicago
Wang, B., Li, H., Yang, R., Zhou, S., Zou, S."Decitabine inhibits the cell growth of cholangiocarcinoma in cultured cell lines and mouse xenografts". Oncology Letters 8, no. 5 (2014): 1919-1924. https://doi.org/10.3892/ol.2014.2499