Moringa oleifera fruit induce apoptosis via reactive oxygen species‑dependent activation of mitogen‑activated protein kinases in human melanoma A2058 cells

  • Authors:
    • Tae Eun Guon
    • Ha Sook Chung
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  • Published online on: May 31, 2017     https://doi.org/10.3892/ol.2017.6288
  • Pages: 1703-1710
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Abstract

The present study was performed to determine the effect of Moringa oleifera fruit extract on the apoptosis of human melanoma A2058 cells. A2058 cells were treated for 72 h with Moringa oleifera fruit extract at 50‑100 µg/ml, and cell viability with apoptotic changes was examined. The involvement of reactive oxygen species (ROS) and mitogen‑activated protein kinases (MAPKs) was examined. It was revealed that Moringa oleifera fruit extract significantly inhibited the cell viability and promoted apoptosis of A2058 cells in a concentration‑dependent manner. Moringa oleifera fruit extract‑treated A2058 cells exhibited increased activities of cleaved caspase‑9 and caspase‑3. It also caused an enhancement of MAPK phosphorylation and ROS production. The pro‑apoptotic activity of Moringa oleifera fruit extract was significantly reversed by pretreatment with the c‑Jun N‑terminal kinase (JNK) inhibitor SP600125, extracellular‑signal‑regulated kinase (ERK) inhibitor PD98058 or ROS inhibitor N‑acetyl‑L‑cysteine. Taken together, Moringa oleifera fruit extract is effective in inducing mitochondrial apoptosis of A2058 cells, which is mediated through induction of ROS formation, and JNK and ERK activation. Moringa oleifera fruit extract may thus have therapeutic benefits for human melanoma A2058 cells.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Guon T and Guon T: Moringa oleifera fruit induce apoptosis via reactive oxygen species‑dependent activation of mitogen‑activated protein kinases in human melanoma A2058 cells. Oncol Lett 14: 1703-1710, 2017
APA
Guon, T., & Guon, T. (2017). Moringa oleifera fruit induce apoptosis via reactive oxygen species‑dependent activation of mitogen‑activated protein kinases in human melanoma A2058 cells. Oncology Letters, 14, 1703-1710. https://doi.org/10.3892/ol.2017.6288
MLA
Guon, T., Chung, H."Moringa oleifera fruit induce apoptosis via reactive oxygen species‑dependent activation of mitogen‑activated protein kinases in human melanoma A2058 cells". Oncology Letters 14.2 (2017): 1703-1710.
Chicago
Guon, T., Chung, H."Moringa oleifera fruit induce apoptosis via reactive oxygen species‑dependent activation of mitogen‑activated protein kinases in human melanoma A2058 cells". Oncology Letters 14, no. 2 (2017): 1703-1710. https://doi.org/10.3892/ol.2017.6288