Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB

  • Authors:
    • Qiuye Ma
    • Yidong Yu
    • Linlin Dai
    • Xuehua Qu
    • Shan Cong
    • Hongsuo Liang
  • View Affiliations

  • Published online on: June 13, 2017     https://doi.org/10.3892/ol.2017.6373
  • Pages: 1939-1942
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Abstract

The present study explored the effect of telomerase reverse transcriptase (TERT) on the growth and apoptosis of fibrosarcoma, and investigated the potential molecular signalling pathways underlying its effect. A plasmid was constructed in order to overexpress TERT and siRNA was used to knockdown TERT. The effect of TERT on fibrosarcoma cells in vitro was studied by performing reverse transcription‑quantitative PCR and western blotting to determine the expression of p53, survivin, caspase‑3, caspase‑7 and PKB. Knockdown of TERT suppressed cell growth, decreased fibrosarcoma volume, decreased survivin and PKB expression, and increased caspase‑3 expression. The results of the present study suggest that TERT regulates the growth of fibrosarcoma in vitro and in vivo, and that this is associated with the expression of caspase‑3 and survivin, in addition to the PKB signalling pathway.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ma Q, Yu Y, Dai L, Qu X, Cong S and Liang H: Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB. Oncol Lett 14: 1939-1942, 2017
APA
Ma, Q., Yu, Y., Dai, L., Qu, X., Cong, S., & Liang, H. (2017). Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB. Oncology Letters, 14, 1939-1942. https://doi.org/10.3892/ol.2017.6373
MLA
Ma, Q., Yu, Y., Dai, L., Qu, X., Cong, S., Liang, H."Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB". Oncology Letters 14.2 (2017): 1939-1942.
Chicago
Ma, Q., Yu, Y., Dai, L., Qu, X., Cong, S., Liang, H."Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB". Oncology Letters 14, no. 2 (2017): 1939-1942. https://doi.org/10.3892/ol.2017.6373