Purine metabolite inosine induced by transforming growth factor‑&beta; promotes epithelial‑mesenchymal transition in colorectal cancer

Hu,Weimin; Cheng,Ling; Zhao,Jian; Wu,Yonghui; Lin,Ying; Yin,Jun

doi:10.3892/ol.2024.14549

Purine metabolite inosine induced by transforming growth factor‑β promotes epithelial‑mesenchymal transition in colorectal cancer

Authors:
- Weimin Hu
- Ling Cheng
- Jian Zhao
- Yonghui Wu
- Ying Lin
- Jun Yin
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Affiliations: Department of Healthcare, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China, Department of Integrated Traditional Chinese and Western Medicine, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China, Department of Chest Surgery, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China, Department of Pathology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China
Published online on: July 1, 2024 https://doi.org/10.3892/ol.2024.14549
Article Number: 416
Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transforming growth factor‑β (TGF‑β) signaling pathway serves a pivotal role in the pathogenesis of colorectal cancer (CRC). However, the specific molecular mechanisms by which the TGF‑β signaling pathway regulates CRC are still not fully understood. In the present study, metabolomics and transcriptomics were used to screen for key metabolites and regulatory genes most related to the regulation of the TGF‑β signaling pathway in CRC. Additionally, reverse transcription‑quantitative PCR, western blotting and Transwell assays were performed to assess the process of epithelial‑mesenchymal transition (EMT). Metabolomics analysis indicated that TGF‑β1 has an impact on purine metabolism, leading to an increase in the purine metabolite inosine. The increase of inosine is essential for facilitating EMT and cell migration in CRC cells. Furthermore, the integrated analysis of metabolomics and transcriptomics data revealed that TGF‑β1 induces the expression of laccase domain‑containing 1 (LACC1), an enzyme involved in the regulation of inosine. Knockdown of LACC1 resulted in a reduction of TGF‑β1‑induced alterations in inosine levels, EMT and cell migration in CRC cells. The results of the present study suggest that the TGF‑β signaling pathway is involved in the regulation of purine metabolism in CRC through the modulation of LACC1 expression. Furthermore, LACC1 appears to influence EMT and cell migration by elevating the levels of the purine metabolite inosine.

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