Open Access

Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and in vitro insights

  • Authors:
    • Shazia Fathima Jaffer Hussain
    • Shahabe Saquib Abullais
    • Kavitha Bottu
    • Logalakshmi Thirumani
    • Iffath Misbah
    • Inamul Hasan Madar
    • Nuha S. Alghamdi
    • Mohmed Isaqali Karobari
  • View Affiliations

  • Published online on: January 3, 2025     https://doi.org/10.3892/ol.2025.14862
  • Article Number: 115
  • Copyright: © Hussain et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the involvement of human papillomavirus (HPV)16 and HPV18 in oropharyngeal malignancies in order to understand the oncogenic mechanisms, and to identify biomarkers for early detection and treatment targets. Given the rising incidence of HPV‑associated cancer, particularly in India, this holds significance in elucidating the molecular basis of these diseases. Structural validation of HPV16 and 18 oncoproteins E6 and E7 was conducted using computational tools, while gene expression profiles related to oral squamous cell carcinoma (OSCC) were analyzed to assess differential expression. The presence of HPV in patient tissue sections was examined using reverse transcription‑PCR. The present study revealed the interactions of HPV16 and 18 E6/E7 oncoproteins, highlighting their role in cancer progression by targeting key tumor suppressors, such as p53 and retinoblastoma protein. Further analysis demonstrated the involvement of HPV16 and 18 E6/E7 oncoproteins in cancer pathways, signaling and telomere regulation, which supports the development of future targeted therapies. HPV16 and 18 E6/E7 represent promising therapeutic targets in OSCC, and provide further insights into potential diagnostic and treatment avenues. The present study contributes to the current understanding of HPV‑associated cancer and innovative strategies in disease management.
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March-2025
Volume 29 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hussain SJ, Abullais SS, Bottu K, Thirumani L, Misbah I, Madar IH, Alghamdi NS and Karobari MI: Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncol Lett 29: 115, 2025.
APA
Hussain, S.J., Abullais, S.S., Bottu, K., Thirumani, L., Misbah, I., Madar, I.H. ... Karobari, M.I. (2025). Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncology Letters, 29, 115. https://doi.org/10.3892/ol.2025.14862
MLA
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29.3 (2025): 115.
Chicago
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29, no. 3 (2025): 115. https://doi.org/10.3892/ol.2025.14862