Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer

  • Authors:
    • Yaning Chen
    • Chenfang Zhu
    • Zhiyou Peng
    • Yalei Dai
    • Yan Gu
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  • Published online on: August 10, 2012     https://doi.org/10.3892/or.2012.1964
  • Pages: 1778-1784
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Abstract

In this study, we investigated the effects of lentivirus (LV)-mediated short hairpin RNA (shRNA) targeting IGF-1R on the growth and lymphangiogenesis of breast cancer. The LV vector effectively delivered the IGF-1R shRNA to MDA-MB‑231 cells, leading to significant reduction of IGF-1R mRNA and protein expression. Infection of MDA-MB-231 cells with LV-IGF-1R shRNA reduced cell growth and migration. Transplantation of MDA-MB-231 cells with suppressed IGF-1R expression in SCID mice reduced tumor growth and lymphangiogenesis. These data collectively suggest that LV-mediated shRNA is an effective way to suppress IGF-1R expression and to inhibit growth and lymphangiogenesis of breast cancer. Specific inhibition of IGF-1R expression with shRNA represents a promising approach for the treatment of breast cancer.
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November 2012
Volume 28 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Chen Y, Zhu C, Peng Z, Dai Y and Gu Y: Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncol Rep 28: 1778-1784, 2012.
APA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., & Gu, Y. (2012). Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer. Oncology Reports, 28, 1778-1784. https://doi.org/10.3892/or.2012.1964
MLA
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28.5 (2012): 1778-1784.
Chicago
Chen, Y., Zhu, C., Peng, Z., Dai, Y., Gu, Y."Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth and lymphangiogenesis in breast cancer". Oncology Reports 28, no. 5 (2012): 1778-1784. https://doi.org/10.3892/or.2012.1964