Open Access

Evaluation of co‑inhibition of ErbB family kinases and PI3K for HPV‑negative head and neck squamous cell carcinoma

  • Authors:
    • Xinyan Geng
    • Shirin Azarbarzin
    • Zejia Yang
    • Rena G. Lapidus
    • Xiaoxuan Fan
    • Yong Teng
    • Ranee Mehra
    • Kevin J. Cullen
    • Hancai Dan
  • View Affiliations

  • Published online on: January 27, 2025     https://doi.org/10.3892/or.2025.8871
  • Article Number: 38
  • Copyright: © Geng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The ErbB/HER family of protein‑tyrosine kinases and PI3K represent crucial targets in the treatment of head and neck squamous cell carcinoma (HNSCC). A combination therapy of afatinib (ErbB inhibitor) and copanlisib (PI3K inhibitor), both Food and Drug Administration‑approved kinase inhibitors, can suppress the growth of human papillomavirus (HPV)‑positive HNSCC. The current study further evaluated the efficacy and clinical potential of this combination therapy for the treatment of HPV‑negative HNSCC in vitro and in vivo. Sulforhodamine B cell viability assay and Annexin V/propidium iodide staining demonstrated that this combination treatment markedly enhanced inhibition of cell viability and reduced cell survival when compared with treatment with either inhibitor alone in two HPV‑negative HNSCC cell lines. Notably, this combination also led to significant inhibition of xenograft tumor growth in mice, without any apparent effects on body weight. Western blot analysis found that copanlisib alone effectively blocked PI3K/Akt signaling but caused upregulation of HER2 and HER3 phosphorylation, as reported in other types of cancer. However, the combination of copanlisib and afatinib completely blocked phosphorylation of the ErbB family (including HER3) and Akt, while also increasing apoptosis. In conclusion, these results suggested that co‑targeting the ErbB family kinases and PI3K using a combination treatment of afatinib and copanlisib may have clinical potential for patients with HPV‑negative HNSCC.
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March-2025
Volume 53 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Geng X, Azarbarzin S, Yang Z, Lapidus RG, Fan X, Teng Y, Mehra R, Cullen KJ and Dan H: Evaluation of co‑inhibition of ErbB family kinases and PI3K for HPV‑negative head and neck squamous cell carcinoma. Oncol Rep 53: 38, 2025.
APA
Geng, X., Azarbarzin, S., Yang, Z., Lapidus, R.G., Fan, X., Teng, Y. ... Dan, H. (2025). Evaluation of co‑inhibition of ErbB family kinases and PI3K for HPV‑negative head and neck squamous cell carcinoma. Oncology Reports, 53, 38. https://doi.org/10.3892/or.2025.8871
MLA
Geng, X., Azarbarzin, S., Yang, Z., Lapidus, R. G., Fan, X., Teng, Y., Mehra, R., Cullen, K. J., Dan, H."Evaluation of co‑inhibition of ErbB family kinases and PI3K for HPV‑negative head and neck squamous cell carcinoma". Oncology Reports 53.3 (2025): 38.
Chicago
Geng, X., Azarbarzin, S., Yang, Z., Lapidus, R. G., Fan, X., Teng, Y., Mehra, R., Cullen, K. J., Dan, H."Evaluation of co‑inhibition of ErbB family kinases and PI3K for HPV‑negative head and neck squamous cell carcinoma". Oncology Reports 53, no. 3 (2025): 38. https://doi.org/10.3892/or.2025.8871